This assay assesses donor- and recipient-specific alleles associated with 15 polymorphic, short tandem repeat (STR) sequences to quantify donor and recipient contributions in post-bone marrow transplant samples. A “DNA fingerprint” is created for the donor and recipient prior to the transplant, and then post-transplant samples are obtained periodically and examined to quantify the amount of donor and recipient STRs in the post-transplant samples. The sensitivity of the assay is approximately 5% donor or recipient cells.
Chimerism by PCR
The CPT codes for this assay will vary depending on whether this is the first time that this patient has been tested (in which case donor(s) and pre-transplant recipient specimens must be tested in addition to a post-transplant sample) or whether this patient has been tested previously (in which case only the post-transplant sample requires testing).
Single donor, pre-, and post-transplant recipient samples:
81265; 81267; G0452-26
Double-donor, pre-, and post-transplant recipient samples:
81265; 81266; 81267; G0452-26
Repeat post-transplant monitoring:
Collect: Peripheral blood or bone marrow: 2-5 mL in ACD (yellow top) or EDTA (purple top) tube
Storage: Room temperature
Remarks: This assay requires three specimens: (1) Pre transplant, (2) Donor, and (3) Post-transplant. Additional volume required if patient’s WBCC is below normal.
Transport: See Standard Shipping Procedures
Van Deerlin VMD, et al. 2000. Clinics Lab Med 20:197-225