Blaine Mooers, Ph.D.

 

Assistant Professor
Biochemistry & Molecular Biology
Director of the Laboratory of Biomolecular Structure and Function
2016-2017 Chair Stanford Synchrotron Radiation Lightsource Users Executive Committee
2016-2017 Member, Stanford Synchrotron Radiation Lightsource Scientific  Advisory Committee
2016-2017 Member, Linac Coherent Light Source Users Executive Committee

Ph.D., Oregon State, 1997

Phone: (405) 271-8300

E-mail: blaine-mooers@ouhsc.edu

 Mailing Address:
 975 N.E. 10th, BRC468
 Oklahoma City, OK  73104

Mooers Lab Members

Dr. Chris Fennell, visiting seminar on 23 May 2017 about oculus rift and molecular graphics. Chris is an excellent scientist and an excellent artist. This seminar will be a visual treat!

Molecular Graphics Workshop, 15 Feb. 2017 at Northeastern State University  Useful as a self-directed tutorial on how to get up and running with PyMOL in less than one hour. Can download as a pdf.

Molecular Graphics Course Related Links

Crystallography & Molecular Graphics Links on LBSF website

Easy PyMOL with horizontal scripts

Easy PyMOL You Tube Videos

PyMOL for Windows, free download site

PyMOL install instructions

Small Angle Scattering Links

Oklahoma COBRE in Structural Biology

5th Oklahoma Symposium on Structural BiologyJune 15 , 2017

Stephenson Cancer Center at OUHSC

OU Supercomputing Center for Education and Research

Laboratory of Biomolecular Structure and Function (LBSF)

Oklahoma Structural Biology Nexus

SSRL User Executive Committee

SSRL/LCLS User Meeting, September 27-29, 2017



Structural biology of messenger RNA editing in the mitochondria of trypanosome

Uridine (U) insertion/deletion editing in trypanosomes is an extensive post-transcriptional process that corrects the coding sequence of most mitochondrial mRNAs.  This editing is required for the subsequent expression of several mitochondrial proteins.  The number of Us that are inserted far exceeds that number that are deleted; there is a net increase in the number of codons after editing.  An enzyme cascade does the editing in the mitochondrion.  The editing reactions are directed by a number of different guide RNAs.  Each guide RNA has the sequence complement of a fragment of the final edited mRNA sequence. Consequently, much of the genetic information for the final RNA transcript comes from both its corresponding gene and the genes for the set of guide RNAs that direct its editing.  In other words, the genetic information flows from DNA to RNA along multiple parallel pathways.  This is an interesting variation of the central paradigm of molecular biology that information flows along one pathway from DNA→RNA→protein.  The evolutionary basis for such a complex and expensive system of information flow is still unclear. 

Our goal is to obtain a rigorous description of the structural biology of this type of RNA editing to improve our understanding of its evolutionary basis, clarify the relationship between this type of RNA editing and other types of RNA editing, and to provide a structural basis of the design of better drugs to fight infections with trypanosomes which threaten 600 million people worldwide. 


 

Selected Publications (*corresponding author, pdb codes from structures determined in my lab or by me):  [MyNCBI Bibliography][Google Scholar Bibliography] [Search Pubmed for Mooers-BH]

  • Mooers, B. H. M.* (published on-line 2 August 2016) Simplifying and enhancing the use of PyMOL with horizontal scripts. Protein Sci. 25(10):1873-82. PMID: 27488983
  • Cruz-Reyes, J.,  Mooers, B.H.M.,  Abu-Adas, Z., Kumar, V., & Gulati, S. (published on-line 6 June 2016)  DEAH-RHA helicase• Znf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes. RNA & Disease 3. pii: e1336.
  • Mooers, B. H. M.* (2016) Direct-methods structure determination of a trypanosome RNA-editing substrate fragment with translational pseudosymmetry. Acta Cryst. D72, 477-487. doi: 10.1107/S2059798316001224. 5AD6.
  • Kumar, V., Madina, B.R., Gulati, S., Vashishti, A.A., Kanyumbu, C., Pieters, B., Shakir, A., Wohlschlegel, J.A., Read, L.K., Mooers, B.H.M. & Cruz-Reyes, J. (2016) REH2C helicase and GRBC subcomplexes may base pair through mRNA and small guide RNA in kinetoplastid editosomes.  J. Biol. Chem. 291,5753-64. doi: 10.1074/jbc.M115.708164
  • Gu, X., Mooers, B.H.*, Thomas, L.M., Malone, J., Harris, S.M., and Schroeder, S.J.* (2015) Structures and energetics of four adjacent G·U pairs that stabilize an RNA helix. J. Phys. Chem. B.  119,13252-61. doi: 10.1021/acs.jpcb.5b06970. PMID: 26425937. PDB-code: 4PCO.
  • Terzyan, S.S., Burgett, A.W., Heroux, A., Smith, C.A., Mooers B.H., and Hanigan, M.H. (2015) Human Gamma-glutamyl transpeptidase 1: structures of the free enzyme, inhibitor-bound tetrahedral transition states and glutamate bound enzyme reveal novel movement within the active site during catalysis. J Biol Chem. 290,17576-86. doi: 10.1074/jbc.M115.659680. PMID: 26013825.
  • Madina, B.R., Kumar, V., Mooers, B.H.M., and Cruz-Reyes*, J. (2015) Native variants of the MRB1 complex exhibit specialized functions in kinetoplastid RNA editing. PLoS One 10(4):e0123441. doi: 10.1371/journal.pone.0123441. PMID: 25928631.
  • Mooers, B.H.M.* (2015) RNA Fusions in crystallographic studies of double-stranded RNA from trypanosome RNA editing. Methods in Mol. Biol. 1240, 191-216. doi:10.1007/978-1-4939-1896-6_14. PMID: 25352146.
  • Criswell, A. and Mooers, B.H.M.* (2015) Structural studies of a double-stranded RNA trypanosome RNA editing by small-angle X-ray scattering. Methods in Mol. Biol. 1240, 191-216. doi: 10.1007/978-1-4939-1896-6_13. PMID: 25352145.
  • Madina, B.R., Kumar, V., Metz, R., Mooers, B.H.M. Bundschuh, R., and Cruz-Reyes, J. (2014). Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition. RNA 20, 1142-52. doi:10.1261/rna.044495.114. PMID: 24865612.
  • Ding, J., Mooers, B.H.M., Zhang, Z., Kale, J., Falcone, D., McNichol, J., Huang, B., Zhang, X.C., Xing, C., Andrews, D.W., and Lin, J. (2014). After embedding in membranes antiapoptotic Bcl-XL protein binds both Bcl-2 homology region 3 and helix 1 of proapoptotic Bax protein to inhibit apoptotic mitochondrial permeabilization. J. Biol. Chem. 289, 11873-96. doi:10.1074/jbc.M114.552562. PMID: 24616095.
  • Sheikh, M.O., Schafer, C.M., Powell, J.T., Rodgers, K.K., Mooers, B.H.M., and West, C.M. (2014) Glycosylation of Skp1 affects its conformation and promotes binding to a model F-box protein. Biochemistry 53, 1657-69. doi:10.1021/bi401707y. PMID: 24506136.
  •  West, M.B., Chen, Y., Wickham, S., Heroux, A., Cahill, K., Hanigan, M.H., and Mooers, B.H.M.* (2013)Novel insights into eukaryotic γ-glutamyl transpeptidase 1 from the crystal structure of the glutamate-bound human enzyme. J. Biol. Chem. 288, 31902-31913. doi:10.1074/jbc.M113.498139. PMID: 24047895. PDB-codes: ‎4GDX, 4GG2
  •  Mooers, B.H.M.* (2013)Structural studies of U-insertion/deletion RNA editing in trypanosomes. In Maas, S. (ed) RNA Editing: Current Research and Future Trends. Caister Academic Press, Norfolk. p. 91-124.
  • Venkatramani, L., Johnson, E.S., Kolavi, G., Air, G.M., Brouillette W.J.*, Mooers B.H.M.* (2012) Crystal structure of a new benzoic acid inhibitor of influenza neuramidase bound with a new tilt induced by overpacking sub-site C6. BMC Struct Biol.12:7. doi: 10.1186/1472-6807-12-7. PMID: 22559154. PDB-Code: 4DGR.
  • Mooers, B.H.M.* & Singh, A. (2011) The crystal structure of an oligo(U):pre-mRNA duplex from a trypanosome RNA editing substrate. RNA 17, 1870-83.  doi: 10.1261/rna.2880311. PMID; 21878548. PDB-Code: 3ND3, 3ND4.
  • Mooers, B.H.M., Baase, W.A., Wray, J.W., & Matthews, B.W.  (2009)  Contributions of all 20 amino acids at site 96 to the stability and structure of T4 lysozyme.  Protein Sci. 18, 871-880.  PDB-Codes: 3C7Y, 3C8R, 3C7W, 3C7Z, 3C80, 3C81, 3C82, 3C8Q, 3C83, 3C8R, 3C8S, 3CDO, 3CDQ, 3CDR, 3CDT, 3CDV, 3FI5.
  • Mooers, B.H.M., Tronrud, E.D., & Matthews, B.W. (2009)  Evaluation at atomic resolution of the role of strain in destabilizing the temperature-sensitive T4 lysozyme mutant Arg 96 --> His. Protein Sci. 18, 863-870. doi: 10.1002/pro.93. PMID:19384984.  PDB-Codes: 3F8V, 3F9L, 3FA0, 3FAD
  • Mooers, B.H.M.* (2008)  Crystallographic studies of DNA and RNA. Methods 47, 168-176. doi: 10.1016/j.ymeth.2008.09.006. PMID: 18848992.
  • Mooers, B.H.M.*  & Matthews, B. W.  (2006)  Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme. Acta Cryst. D 62, 165-76.  *Corresponding author.doi:10.1107/S0907444905037212PMID: 16421448. PDB-Codes: 2ANX, 2ANV
  • Mooers,B.H.M., Logue, J.S. & Berglund, J.A. (2005)  The structural basis of myotonic dystrophy from the crystal structure of CUG repeats. Proc. Nat. Acad. Sci., USA 102, 16626-16631. 
     doi: 10.1073/pnas.0505873102.  PMID: 16269545.
  • Mooers, B.H.M. & Matthews, B.W. (2004) Use of an ion binding site to by-pass the 1000 atom limit to ab initio structure determination by direct methods. Acta Cryst. D 60, 1726-1737. doi:10.1107/S0907444904017020PMID: 15388918.  PDB-Codes: 1SWZ, 1SX7, 1SX2, and 1SWY
  • Sagermann, M., Gay, L., Mooers, B.H.M., & Matthews, B.W.  (2004)  Relocation or duplication of the helix A sequence of T4 lysozyme causes only modest changes in structure but can increase or decrease the rate of folding.Biochemistry 33, 1296-301.
  • Mooers, B.H.M., Datta, D., Zollars, E., Mayo, S.L., & Matthews, B. W. (2003) Repacking the core of T4 lysozyme by automated design. J. Mol. Biol.  332, 741-756. PDB-Codes: 1PQM, 1PQO, 1PQK, 1PQJ, 1PQI, 1PQD, 1P7S, 1P46, 1P6Y, 1P64, 1P46, 1P3N, 1P37, 1P36, 1P2R, 1P2L