Lester A. Reinke

 

Professor, Departent of Pharmaceutical Sciences
Professor, Department of Cell Biology


EDUCATION

B.S., Pharmacy, University of Nebraska,
M.S., Medicinal Chemistry, University of Nebraska,
Ph.D., Biomedicinal Chemistry, University of Nebraska Medical Center


RESEARCH SUMMARY

Alcoholic liver injury: Although a relationship between chronic alcohol abuse and liver injury has been known for centuries, the mechanisms through which alcohol damages the liver are not clearly established. Alcohol-induced oxidative stress is thought to have a major role in this toxicity. Chronic alcohol exposure induces several forms of hepatic cytochrome P-450, especially CYP 2E1, and this isozyme is considered to be an important source of oxygen radicals. Alcohol also promotes absorption of bacterial endotoxin from the gastrointestinal tract, which activates liver macrophages to increase production of nitric oxide and several inflammatory cytokines. These and related changes in liver function are the major focus of research in my laboratory.

• Free Radicals and Spin Trapping: Free radicals have been implicated in the pathogenesis of a number of disease states. Because most free radicals are highly reactive and have extremely short half-lives, their roles in tissue injury have been difficult to study directly. Many radicals can be trapped with suitable spin trapping agents, and the resulting spin adducts can be detected with electron paramagnetic resonance (EPR) spectroscopy. My laboratory is exploring novel biological applications of the spin trapping technique, and its potential for the study of free radical toxicology.

SELECTED PUBLICATIONS

Kotake Y, Moore DR and Reinke LA. Online continuous monitoring of in vivo hepatic nitric oxide generation through biliary efflux. Abstracts, International EPR Workshop on In Vivo EPR and Related Studies, Hanover NH, September 14-18, 1998.

Kotake Y, Moore DR, Sang H and Reinke LA. Continuous monitoring of in vivo hepatic nitric oxide formation using EPR trapping analysis in biliary flow. Abstracts, Fifth Annual Meeting of the Oxygen Society, Washington, November 19-23, Free Rad. Biol. Med. 25 (Supplement 1): S105, 1998.

Reinke LA, Moore DR, Sang H, Janzen EG and Kotake Y. Phenyl N-t-butylnitrone hydroxylation by cytochrome P-450 and hydroxyl radicals. Abstracts, Sixth Annual Meeting of the Oxygen Society, New Orleans, November 18-22, Free Rad. Biol. Med. 27 (Supplement 1): S133, 1999.

Reinke LA, Moore DR, Taylor JM and Koop DR. Gadolinium chloride decreases cytochrome P-450 levels in rat liver and interferes with EPR measurements in bile. Abstracts, Seventh Annual Meeting of the Oxygen Society, San Diego, November 16-20, Free Rad. Biol. Med. 29 (Suppl.1): S120, 2000.

Reinke LA and Nanji AA. Pronounced hepatic free radical formation precedes pathological liver injury in ethanol-fed rats. Abstracts, 42nd Annual Meeting of the Society of Toxicology, Salt Lake City, March 9-13, 2003.

Gallucci RM, Sloan DK, O'Dell SK and Reinke LA. Differential expression of liver IL-6Rα in female vs. male ethanol consuming rats. Abstracts, American Association of Immunology, Denver, March 3-13, 2003.

Gallucci RM, Sloan DK, O'Dell SJ and Reinke LA. Differential expression of liver IL-6R-alpha in Female vs. male ethanol consuming rats. Abstracts, 27th Annual Meeting of the Research Society on Alcoholism, Vancouver, June 26-30, Alcohol. Clin. Exp. Res. 28 (Supplement), 35A, 2004.

MAILING ADDRESS

University of Oklahoma Health Sciences Center
College of Pharmacy, Department of Pharmaceutical Sciences
P.O. Box 26901
Oklahoma City, OK 73126-0901
Phone: (405) 271-6593, ext. 47246
Fax: (405) 271-7505

Lester-Reinke@ouhsc.edu

CV - Lester Reinke, Ph.D.