Muralidharan JayaramanJayaramanMuralidharan_2015_B_edit

Assistant Professor of Research

EDUCATION

BAGR, University of Agricultural Sciences, Bangalore, Karnataka
MAGR, University of Agricultural Sciences, Bangalore, Karnataka
Ph.D., Pondicherry University, Pondicherry, Pondicherry

RESEARCH SUMMARY

Ovarian cancer is the most lethal gynecological cancer, with anticipated death in 2017 at colossal 14,080 women. Treatment for ovarian cancer starts by resection of the tumor with adjuvant treatment using genotoxic drugs. Recurrence is common due to chemo-resistance to these DNA damaging drugs. DNA damage caused due to the drugs are repaired by a host of different mechanisms. One of them is non-homologous end-joining mechanism which recruits 53BP1 to the damaged site. The 53BP1 protein recruits a multitude of proteins including MUM1 or EXPAND1 (Gene ID: 84939). MUM1 role in DNA repair or cell cycle checkpoint arrest is not clear, but it has a PWWP domain that is predicted to bind with histone H4. Moreover, MUM1 gene DNA fragments were found at higher concentration in platinum resistant patient serum, hence understanding MUM1 should pave a way to use it as a chemo-resistance biomarker.

Biomarkers that aid in early diagnosis with the least invasive method would reduce anxiety as well as reach more people rather than advanced highly sophisticated diagnosis. The biomarker molecules under study are circulating DNA in patient serum samples, long non-coding RNA in exosomes of urine and serum, biomarkers of early-life disease as predictive markers for predisposition of ovarian cancer and microRNA in serum samples of ovarian cancer patients.

Stephenson Cancer center (SCC) COBRE established Biospecimen Pathology, Immunochemistry and Histology and Small animal imaging core facilities equipped with state-of-the-art high-throughput technology. Few of them are Operetta for quantitative immunostaining, Surescan scanner for miRNA microarray scanning, Nanoprint for lysate/antibody imprinting, Janus for automated liquid handling, XFe analyzer for extracellular flux analysis, Droplet digital PCR for absolute quantification of genes, Envision for high-throughput plate reader, Genepix for fluorescent scanner, Gelcount for counting colonies on any plate format, Aperio tissue microarray (TMA) lab software for evaluation of immunohistochemistry stained TMA, TMA arrayer for TMA construction and Leica Bond III for immunohistochemistry staining. Apart from my research, I am the Director of Operations for the research core facilities, with expertise that include high-content high-throughput analysis using fluorescent-labelled proteins, gene mutation analysis and protein biochemistry.


SELECTED PUBLICATIONS

1. Jayaraman M, Radhakrishnan R, Mathews CA, Yan M, Husain S, Moxley KM, Song YS, Dhanasekaran DN. Genes & cancer, 2017. Identification of novel diagnostic and prognostic miRNA signatures in endometrial cancer. 8(5-6):566-576.

2.  Chakraborty PK, Mustafi SB, Xiong X, Dwivedi SKD, Nesin V, Saha S, Zhang M, Dhanasekaran D, Jayaraman M, Mannel R, Moore K, McMeekin S, Yang D, Zuna R, Ding K, Tsiokas L, Bhattacharya R, Mukherjee P. Nat Commun, 2017. MICU1 drives glycolysis and chemoresistance in ovarian cancer. 8:14634. PMID: 28530221

3. Ha JH, Ward JD, Radhakrishnan R, Jayaraman M, Song YS, Dhanasekaran DN. Oncotarget, 2016. Lysophosphatidic acid stimulates epithelial to mesenchymal transition marker Slug/Snail2 in ovarian cancer cells via Gαi2, Src, and HIF1α signaling nexus. 7(45):72845-72859 PMID: 27166196

4. Gomathinayagam R, Muralidharan J, Ha JH, Varadarajalu L, Dhanasekaran DN. Genes & cancer, 2014. Hax-1 is required for Rac1-Cortactin interaction and ovarian carcinoma cell migration. 5(3-4):84-99. PMID: 25053987

5. Singhal A, Jayaraman M, Dhanasekaran DN, Kohli V. Critical reviews in oncology/hematology, 2012. Molecular and serum markers in hepatocellular carcinoma: predictive tools for prognosis and recurrence. 82(2):116-40. PMID: 21680198

6. Grabowska D, Jayaraman M, Kaltenbronn KM, Sandiford SL, Wang Q, et al. Neuroscience, 2008. Postnatal induction and localization of R7BP, a membrane-anchoring protein for regulator of G protein signaling 7 family-Gbeta5 complexes in brain. 151(4):969-82. PMID: 18248908

7. Radhika V, Proikas-Cezanne T, Jayaraman M, Onesime D, Ha JH, et al. Nature chemical biology, 2007. Chemical sensing of DNT by engineered olfactory yeast strain. 3(6):325-30. PMID: 17486045

8. Jayaraman M, Radhika V, Bamne MN, Ramos R, Briggs R, et al. Biotechnology progress, 2005. Engineered Saccharomyces cerevisiae strain BioS-OS1/2, for the detection of oxidative stress. 21(5):1373-9. PMID: 16209540

9. Muralidharan J, Suguna K, Surolia A, Surolia N. Journal of biomolecular structure & dynamics, 2003. Exploring the interaction energies for the binding of hydroxydiphenyl ethers to enoyl-acyl carrier protein reductases. 20(4):589-94. PMID: 12529157

10. Muralidharan J, Jayachandran, S., 2003; Physicochemical properties of exopolysaccharide produced by a marine biofouling bacteria, Vibrio alginolyticus. Process Biochemistry, 38:841-847.



MAILING ADDRESS

Stephenson Cancer Center
University of Oklahoma Health Sciences Center
975 NE 10th Street, BRC 1401
Oklahoma City, OK, 73104

Muralidharan-Jayaraman@ouhsc.edu