BCR-ABL1, Qualitative with Reflex to Quantitative, Major

Specimen Collection, Storage, and Shipping

Test Information:

The test uses reverse transcription and PCR to detect BCR-ABL1 fusion genes resulting from the t(9;22)(q34.12q11.23) (Philadelphia chromosome) translocation involving major (p210), minor (p190), or mu (p230) bcr breakpoints. The t(9;22) is found in chronic myelogenous leukemias (CML) and a subset of acute lymphoblastic leukemia (ALL) patients (about 25% of adult and 5% of childhood ALLs). The test can be used to screen for the BCR-ABL1 fusion and distinguishes between major, minor or mu breakpoints. The test is not designed to monitor patients for residual disease. If the sample is positive for the major (p210) breakpoint, it will be tested for quantification. Please see BCR-ABL1, Quantitative, Major for test details. If the sample is positive for the minor (p190) breakpoint, the quantitative assay can be ordered separately, if desired.

Requisition Code:

BCR-ABL1, Qualitative w/Reflex to Quantitative, Major (presence/absence, major, minor, mu)

CPT Code:

81206; 81207; 81208; G0452-26 (x3)

Turn Around Time:

5-7 days

Specimen Requirements:

Collect:         Peripheral blood: 2-5 mL in EDTA (purple top) tube

                      Bone marrow: 2-5 mL in ACD (yellow) or EDTA (purple) tube

Storage:        Refrigerate

Remarks:      Specimens must be received within 72 hours from collection due to instability of RNA.
                       WBCC is required to accompany specimen.

Transport:      See Standard Shipping Procedure

Reference:

Cross NC, Melo JV, Feng L, Goldman JM. An optimized multiplex polymerase chain reaction (PCR) for detection of BCR-ABL fusion mRNAs in haematological disorders. Leukemia. 1994 Jan;8(1):186-9.