Bone Marrow Engraftment Monitoring by PCR

Specimen Collection, Storage, and Shipping

Test Information:

This assay is used to determine the percentage of donor and recipient cells after allogeneic stem cell transplantation. Samples for the donor and recipient prior to the transplant, and post-transplant samples are used to quantify donor and recipient contributions. The 15 polymorphic, short tandem repeat (STR) sequences used include the 13 core CODIS (Combined DNA Index System) loci for human identification. Percentages <5% are below our limit of quantification.

Requisition Code:

Chimerism by PCR

CPT Codes:

CPT codes depend on whether this is the first time testing (in which case donor(s) and pre-transplant recipient specimens must be tested in addition to a post-transplant sample) or follow-up testing (in which case only the post-transplant sample requires testing).

Initial post-transplant monitoring:

Single donor, pre-, and post-transplant recipient samples:

         81265; 81267; G0452-26 (x2)

Double-donor, pre-, and post-transplant recipient samples:

         81265; 81266; 81267; G0452-26 (x3)

Repeat post-transplant monitoring:

         81267; G0452-26

Twins determination:

         81265; G0452-26

Turn Around Time:

5-7 days

Specimen Requirements:

Collect:          Peripheral blood: 2-5 mL in ACD (yellow top), EDTA (purple top), CPD (red) tube

                        Bone marrow: 2-5 mL in ACD (yellow top) or EDTA (purple top) tube

                        Cord blood: 2-15 mL in ACD (yellow top) or CPD (red) tube

Storage:         Room temperature

Remarks:       This assay requires three specimens: (1) Pre transplant, (2) Donor, and (3) Post-transplant.Additional volume required if patient’s WBCC is below normal. *Extracted DNA must be isolated in a CLIA-certified laboratory. Copy of certification is required to accompany DNA samples.

Transport:     See Standard Shipping Procedures

References:
  1. Van Deerlin VMD and Leonard DGB. Bone marrow engraftment analysis after allogeneic bone marrow transplantation. Clinics in Laboratory Medicine. 2000 Mar;20(1):197-225.