Allison Gillaspy, PhD

Director of the OUHSC Laboratory for Molecular Biology and Cytometry Research

Dr. Gillaspy is the Director of the Laboratory for  Molecular Biology and Cytometry Research at OUHSC ( which is a state of the art facility that specializes in genomic technology and custom DNA sequencing.  We are involved in different aspects of research projects for several PIs on the OUHSC campus as well as other academic institutions across the country.  A major focus for our lab in recent years has been to aid in the integration of next generation sequencing technology, specifically the SOLiD platform from Applied Biosystems, into existing biomedical research. Using this technology we have participated in both clinical and basic science research endeavors with numerous groups interested in a variety of scientific endeavors. Specifically, we have done miRNA profiling for cancer and control patient samples including those for ovarian, colon and pancreatic cancer.  We have also sequenced numerous (>40) whole genomes for bacterial and viral samples that have been representative of laboratory strains as well as direct clinical isolates.  Whole transcriptome studies in rat and mouse cancer cell lines as well as samples from in vivo experiments have also been done as well as sequencing RNA or DNA from model plant systems and other organisms for which no genomic information had been gathered previously.   In addition to the genomic aspect of our lab, we also offer services that are more routine such as plasmid prepping and sequencing, Real Time PCR set up and cycling, proteome analysis, and quantitative/qualitative analysis of samples (DNA, RNA, or protein) using the Agilent BioAnalyzer.

Current Laboratory Personnel:

Jenny Gipson, Research Assistant

Steven Snow, Research Assistant

Selected Publications:

Fisch, K.M., Gillaspy, A.F., Gipson, M., Henrikson, J.C., Hoover, A.R., Jackson, L., Joyner, P.M., Najar, F. Z., Roe, B.A., Sims, P.A., Wägele, H., and Cichewicz, R.H.  2008.  Inducing native-host expression of silent gene clusters encoding for secondary metabolites through small molecule modulation of the fungal epigenome.  Submitted to Fungal Genetics and Biology.   

Robinson, C.M., Shariati, F., Zaitshik, J., Gillaspy, A.F., Dyer, D.W., Chodosh, J.  2008.  Human Adenovirus type 19:  Genomic and bioinformatics analysis of a keratoconjunctivitis isolate. Virus Research. In press.

Barua, S., McKevitt, M., DeGiusti, K., Hamm, E., Larabee, J., Shakir, S., Bryant, K., Koehler, T., Blanke, S., Dyer, D., Gillaspy, A., Ballard, J.  2008.  The Mechanism of Bacillus anthracisIntracellular Germination Requires Multiple and Highly Diverse Genetic Loci. Infection and Immunity.  In press.

Robinson, C.M., Shariati, F., Gillaspy, A.F., Dyer, D.W., Chodosh, J.  2008.  Genomic and bioinformatic analysis of human adenovirus type 37:  New insights into corneal tropism.  BMC Genomics.  9:213-224.

Williams, M.L., Waldbieser, G.C., Dyer, D.W., Gillaspy, A.F., Lawrence, M.L.  2008. Characterization of the rrn operons in the channel catfish pathogen Edwardsiella ictaluri. Journal of Applied Microbiology.  104(6):1790-1796.

Harrison, A., Dyer, D.W., Gillaspy, A., Ray, W.C., Mungur, R., Carson, M.B., Gipson, J., Gipson, M., Johnson, L.S., Lewis, L., Bakaletz, L.O., Munson, R.S., Jr..  2005.  Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae:  comparative study with H. influenzae serotype d, strain KW20.  Journal of Bacteriology.  187(13):4627-36.

Munson, R.S., Jr., Harrison, A., Gillaspy, A., Ray, W.C., Carson, M., Armbruster, D., Gipson, J., Gipson, M., Johnson, L., Lewis, L., Dyer, D.W., Bakaletz, L.O.  2004.  Partial analysis of the genomes of two nontypeable Haemophilus influenzae otitis media isolates.  Infection and Immunity.  72(5):3002-3010.  

Williams, W.A., A. Hendrickson, A.F. Gillaspy, D.W. Dyer and L.A. Lewis. Oligonucleotide analysis by sequential injection before analysis (SIBA) capillary electrophoresis.  2003. Analytical Biochemistry.  313:183-185.

Rohde, K.H., A.F. Gillaspy, M.D. Hatfield, L.A. Lewis and D.W. Dyer. 2002.  Interactions of haemoglobin with the Neisseria meningitidis receptor HpuAB: the role of TonB and an intact proton motive force. Molecular
Microbiology. 43(2):335-354.

Smeltzer, M.S., A.F. Gillaspy.  2000.  Molecular pathogenesis of staphylococcal osteomyelitis. Poultry Science.  79(7):1042-1049.

Blevins, J.S., A.F. Gillaspy, T.M. Rechtin, B.K. Hurlburt and M.S. Smeltzer. 1999.  The staphylococcal accessory regulator (sar) represses transcription of the Staphylococcus aureus collagen adhesin gene (cna) in an agr-independent manner. Molecular Microbiology. 33(2):317-326.

Rechtin, T.M., A.F. Gillaspy, M.A. Schumacher, R.G. Brennen, M.S. Smeltzer and B.K. Hurlburt. 1999. Characterization of the SarA virulence gene regulator of Staphylococcus aureus. Molecular Microbiology. 33(2):307-316.

Gillaspy, A.F., C.Y. Lee, S. Sau, A.L. Cheung and M.S. Smeltzer. 1998. Factors affecting the collagen binding capacity of Staphylococcus aureus.  Infection and Immunity.  66(7):3170-3178.

Gillaspy, A.F., J.M. Patti, F.L. Pratt, Jr., J.J. Iandolo and M.S. Smeltzer. 1997.  TheStaphylococcus aureus collagen adhesin encoding gene (cna) is within a discrete genetic element. Gene. 196:239-248.

Gillaspy, A.F., J.M. Patti and M.S. Smeltzer. 1997.  Transcriptional regulation of theStaphylococcus aureus collagen adhesin gene (cna).  Infection and Immunity.  65:1536-1540.

Smeltzer, M.S., A.F. Gillaspy, F.L. Pratt, Jr., M.D. Thames and J.J. Iandolo.  1997.  Prevalence and chromosomal map location of Staphylococcus aureus adhesin genes.  Gene.  196:249-259.

Smeltzer, M.S., A.F. Gillaspy, F.L. Pratt, Jr., M.D. Thames. 1997.  Comparative evaluation of the use of cna, fnbA, fnbB and hlb for genomic fingerprinting in the epidemiological typing ofStaphylococcus aureus.  Journal of Clinical Microbiology.  35:2444-2449.

Smeltzer, M.S., F.L. Pratt, Jr., A.F. Gillaspy and L.A. Young.  1996.  Genomic fingerprinting for epidemiological differentiation of Staphylococcus aureus clinical isolates.  Journal of Clinical Microbiology.  34:1364-1372.

Gillaspy, A.F., S.G. Hickmon, R.A. Skinner, J.R. Thomas, C.L. Nelson and M.S. Smeltzer. 1995. Role of the accessory gene regulator (agr) in the pathogenesis of staphylococcal osteomyelitis.  Infection and Immunity. 63:3373-3380.